{"id":134,"date":"2025-06-24T05:35:07","date_gmt":"2025-06-24T05:35:07","guid":{"rendered":"https:\/\/genoinfocore.crc.nycu.edu.tw\/?p=134"},"modified":"2025-07-01T01:41:53","modified_gmt":"2025-07-01T01:41:53","slug":"%e5%b0%88%e5%ae%b6%e7%b0%a1%e4%bb%8b%ef%bc%9a%e8%91%a3%e5%bb%ba%e5%84%84","status":"publish","type":"post","link":"https:\/\/genoinfocore.crc.nycu.edu.tw\/?p=134","title":{"rendered":"\u5c08\u5bb6\u7c21\u4ecb\uff1a\u8463\u5efa\u5104"},"content":{"rendered":"\n
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\u9ede\u6211\u9810\u7d04<\/a><\/div>\n<\/div>\n\n\n\n

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<\/p>\n\n\n\n

\u7814\u7a76\u9818\u57df\uff1a<\/strong>
Genomics, Microarray, Bioinformatics, NGS, Computer programming<\/p>\n\n\n\n

\u5b78\u6b77\uff1a<\/strong>
\u570b\u7acb\u81fa\u7063\u5927\u5b78\u690d\u7269\u7cfb\u5b78\u58eb
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\u570b\u7acb\u967d\u660e\u5927\u5b78\u5fae\u751f\u7269\u66a8\u514d\u75ab\u5b78\u7814\u7a76\u6240\u535a\u58eb<\/p>\n\n\n\n

\u7d93\u6b77\uff1a<\/strong>
\u967d\u660e\u5927\u5b78\u69ae\u967d\u57fa\u56e0\u9ad4\u4e2d\u5fc3
1. \u7531cDNA\u5eab\u7814\u767c\u967d\u660e\u57fa\u56e0\u9ad4\u4e2d\u5fc3\u7b2c\u4e00\u7247cDNA\u751f\u7269\u6676\u7247\uff0c\u4e3b\u5c0e\u6676\u7247\u683c\u5f0f\u8a2d\u8a08\u3001\u8f09\u9ad4\u8a2d\u8a08\u53ca\u91cf\u7522\u8a55\u4f30\u3002
2. \u958b\u767c\u8a2d\u8a08\u9069\u7528\u65bc\u9ad8\u6b67\u7570\u5ea6\u8cc7\u6599\u4e4b\u57fa\u56e0\u7d44\u5206\u6790\u6f14\u7b97\u6cd5\uff08eGSA\uff09\uff0c\u89e3\u6c7a\u764c\u75c7\u57fa\u56e0\u9ad4\u7814\u7a76\u9762\u81e8\u9ad8\u6b67\u7570\u5ea6\u7684\u554f\u984c\u3002
3. \u63d0\u4f9b\u57fa\u56e0\u9ad4\u4e2d\u5fc3\u5408\u4f5c\u7814\u7a76\u4eba\u54e1\u5fae\u9663\u5217\u6676\u7247\u8cc7\u6599\u5206\u6790\u670d\u52d9\uff0c\u670d\u52d9\u5167\u5bb9\u5305\u62ec\u7d71\u8a08\u5206\u6790\uff08R, Partek, TM4 MeV\uff09\uff0c\u8abf\u63a7\u7db2\u8def\uff08Ingenuity, Cytoscape\uff09\u53ca\u529f\u80fd\u8a3b\u89e3\u3002
4. \u5fae\u9663\u5217\u6676\u7247\u57fa\u790e\u8cc7\u6599\u8655\u7406\u3001\u57fa\u56e0\u7d44\u5206\u6790\u53ca\u57fa\u56e0\u7db2\u8def\u5206\u6790\u3002
5. \u63d0\u4f9b\u57fa\u56e0\u4e2d\u5fc3\u5408\u4f5c\u7814\u7a76\u4eba\u54e1\u5fae\u9663\u5217\u53ca\u6b21\u4e16\u4ee3\u5b9a\u5e8f\u8cc7\u6599\u5206\u6790\u670d\u52d9\uff0c\u76ee\u524d\u5408\u4f5c\u6848\u4f8b\u5305\u62ec\u764c\u75c7\u6a19\u5e5f\uff0cSNP\u5206\u6790\uff0c\u907a\u50b3\u75be\u75c5\u81f4\u75c5\u7a81\u8b8a\uff0c\u81ea\u9ad4\u7a81\u8b8a\u5075\u6e2c\uff0c\u57fa\u56e0\u9078\u64c7\u6027\u526a\u63a5\u5206\u6790\uff0c\u5fae\u751f\u7269\u83cc\u76f8\u5206\u6790\u3002
6. \u7814\u767c\u8abf\u7bc0\u4e8b\u4ef6\u57fa\u56e0\u96c6\u5206\u6790\u6f14\u6cd5\uff08Regulatory Event-based Gene Set Analysis\uff09\uff0c\u89e3\u6c7a\u764c\u75c7\u81e8\u5e8a\u7814\u7a76\u4e4b\u9ad8\u5ea6\u6b67\u7570\u5ea6\u554f\u984c\uff0c\u4e26\u4ee5R\u8a9e\u8a00\u958b\u767ceGSA\u5fae\u9663\u5217\u6676\u7247\u5206\u6790\u4e4b\u8edf\u9ad4\u5957\u4ef6\uff0ceGSA-R\u3002
7. \u7814\u767c\u9ad8\u901f\u5e8f\u5217\u6bd4\u5c0d\u6f14\u7b97\u6cd5\uff08hcMSA\uff09\uff0c\u89e3\u6c7aMetagenome \u7814\u7a76\uff0c\u5927\u898f\u6a21\u591a\u5e8f\u5217\u6bd4\u5c0d\u4e4b\u6280\u8853\u74f6\u9838\uff0c\u4e26\u4ee5\u6b64\u6838\u5fc3\u6280\u8853\uff0c\u4ee5C#\u8a9e\u8a00\u958b\u767c\u5206\u6790\u8edf\u9ad4\u5fae\u751f\u7269\u540c\u6e90\u5e8f\u5217\u81ea\u52d5\u5206\u6790\u8edf\u9ad4\u5e73\u53f0\uff08Orthologous Sequence Variation Analysis System, OSVAS\uff09\u3002
8. \u4ee5\u8de8\u5e73\u53f0JAVA\u7a0b\u5f0f\u8a9e\u8a00\u958b\u767cGenome Analyst GUI workflow system\uff0c\u63d0\u4f9b\u8edf\u9ad4\u5c01\u88dd\u6280\u8853\uff0c\u5c07\u5404\u7a2e\u57fa\u56e0\u9ad4\u5206\u6790\u8edf\u9ad4\u7d71\u5408JAVA\u3001R\u3001Python\u3001Web-based \u5de5\u5177\u65bc\u540c\u4e00\u5716\u50cf\u5316\u64cd\u4f5c\u754c\u9762\u4e0b\uff0c\u6253\u7834\u751f\u7269\u8cc7\u8a0a\u5206\u6790\u6280\u8853\u9580\u6abb\uff0c\u4f7f\u975e\u8cc7\u8a0a\u76f8\u95dc\u4eba\u54e1\u7121\u9700\u7a0b\u5f0f\u57fa\u790e\uff0c\u5c31\u53ef\u4ee5\u4f7f\u7528\u5148\u9032\u5206\u6790\u8edf\u9ad4\u3002<\/p>\n\n\n\n

\u5c08\u9577\uff1a<\/strong>
\u7d30\u80de\u57f9\u990a\u53ca\u5206\u5b50\u751f\u7269\u6280\u8853<\/em><\/strong>
\u7d30\u80de\u57f9\u990a\u76f8\u95dc\u64cd\u4f5c\uff0c\u4e00\u822c\u5206\u751f\u64cd\u4f5c\uff08DNA\/RNA isolation, cloning, PCR, RT, real-time quantitative PCR, Southern blot\u2026\uff09\uff0c\u9ad8\u901a\u91cf\u6280\u8853\u64cd\u4f5c\uff08liquid handling, array spotting, automated pipeline\uff09\uff0c\u57fa\u672c\u986f\u5fae\u93e1\u8853\uff08Immune florescence staining, microscope operation\uff09\u3002<\/p>\n\n\n\n

\u57fa\u56e0\u9ad4\u5b78\u6280\u8853<\/em><\/strong>
\u57fa\u56e0\u8868\u9054\u5e8f\u5217\u5206\u6790\u6cd5\uff08SAGE\uff09\uff0c\u5fae\u9663\u5217\u6676\u7247\u8a2d\u8a08\u3001\u751f\u7522\u53ca\u5be6\u9a57\u64cd\u4f5c\uff0c\u5404\u7a2e\u5546\u7528\u6676\u7247\u5e73\u53f0\u53ca\u6b21\u4e16\u4ee3\u5b9a\u5e8f\u5206\u6790\u3002<\/p>\n\n\n\n

\u751f\u7269\u8cc7\u8a0a\u6280\u8853<\/em><\/strong>
\u5fae\u9663\u5217\u6676\u7247\u53ca\u6b21\u4e16\u4ee3\u5b9a\u5e8f\u8cc7\u6599\u5206\u6790\uff1a\u8cc7\u6599\u524d\u8655\u7406\u3001\u54c1\u8cea\u5206\u6790\u53ca\u7d71\u8a08\u5206\u6790\uff0c\u57fa\u56e0\u529f\u80fd\u8a3b\u89e3\u53ca\u57fa\u56e0\u7d44\u8c50\u5ea6\u5206\u6790\u3002\u591a\u91cd\u5e8f\u5217\u6bd4\u5c0d\u5206\u6790\uff08Multiple Sequence Alignment\uff09\uff0c\u5f15\u5b50\u53ca\u63a2\u91dd\u5e8f\u5217\u8a2d\u8a08\u53ca\u6f14\u7b97\u6cd5\u958b\u767c\u3002<\/p>\n\n\n\n

\u7a0b\u5f0f\u8a9e\u8a00\u53ca\u958b\u767c\u8edf\u9ad4<\/em><\/strong>
R:event-based Gene Set Analysis Package
C#: Orthologous Sequence Variation Analysis System Ver. 1
JAVA:
1. Orthologous Sequence Variation Analysis System Ver. 2
2. Genome Analyst GUI Workflow System
3. Other language skill: PHP, SQL, VB and VBA<\/p>\n\n\n\n

\u7814\u7a76\u6210\u679c\uff1a<\/strong>
1. Gelfanov, Y.G. Lai, V. Gelfanova, J.Y. Dong, J.P. Su and N.S. Liao Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells. J Immunol 155. 76-82. 1995.
2. Y. Tung, W.N. Lian, Y.J. Chen, C.H. Lin., Changes of transcriptome profiles during hepatic differentiation. poster in 9th HUGO\u2019s Human Genome Meeting, Shanghai, China, 2002.
3. Y. Tung, C.H. Lin, \u529f\u80fd\u57fa\u56e0\u9ad4, \u5206\u5b50\u6aa2\u9a57, 303-346, 2003.
4. Y. Tung, H.F. Haung, W.N. Lian, H.W. Wong, C.H. Lin., Dynamic analyses of gene expression profiles during hepatic differentiation. poster in 11th HUGO\u2019s Human Genome Meeting, Kyoto, Japan, 2005.
5. H. Jen, T.P. Yang, C.Y. Tung, S.H. Su, C.H. Lin, M.T. Hsu and H.W. Wang, Signature Evaluation Tool (SET): a Java-based tool to evaluate and visualize the sample discrimination abilities of gene expression signatures. BMC Bioinformatics. 2008; 9: 58.
6. Y. Tung, C.H. Jen, M.T. Hsu, H.W. Wang and C.H. Lin, A novel regulatory event-based gene set analysis method for exploring global functional changes in heterogeneous genomic data sets. BMC Genomics 2009, 10:26.
7. C Cheng, C.F. Chen, Y.J. Jong, Y. Tung, Y.J. Chen, W.W. Sheng, L.H. Li, S.F. Tsai, M.H. Tsou, Skye H. Cheng, C.M. Chen, Andrew T. Huang, C.H. Lin, M.T. Hsu. (2011) Comparison of genome aberrations between early-onset and late-onset breast cancer. Breast Cancer Cells \/ Book 5\u2033, ISBN , 979-953-307-184-7
8. H. Wang, T.H. Hsieh, S.Y. Huang, G.Y. Chau, C.Y. Tung, C.W. Su, J.C. Wu, Forfeited hepatogenesis program and increased embryonic stem cell traits in young hepatocellular carcinoma (HCC) comparing to elderly HCC. BMC Genomics. 2013 Oct 26;14:736.
9. S. Hsu, C.Y. Tung, C.Y. Yang, C.H. Lin, Response to Stress in Early Tumor Colonization Modulates Switching of CD133-Positive and CD133-Negative Subpopulations in a Human Metastatic Colon Cancer Cell Line, SW620. PLoS One. 2013;8(4):e61133.
10. Y.Yang, R.H. Lu, C.H. Lin, C.H. Jen, C.Y. Tung, S.H. Yang,J.K. Lin, J.K. Jiang , C.H. Lin. Single nucleotide polymorphisms associated with colorectal cancer susceptibility and loss of heterozygosity in a Taiwanese population. PLoS One. 2014 Jun 26;9(6):e100060.
11. C. Liang, C.Y. Yang, J.Y. Tseng, H.L. Wang, C.Y. Tung, H.W. Liu, C.Y. Chen, Y.C. Yeh, T.Y. Chou, M.H. Yang, J. Whang-Peng, C.H. Lin.ABCG2 Localizes to the Nucleus and Modulates CDH1 Expression in Lung Cancer Cells. Neoplasia. 2015 Mar;17(3):265-78.<\/p>\n\n\n\n

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