{"id":230,"date":"2025-06-25T02:29:12","date_gmt":"2025-06-25T02:29:12","guid":{"rendered":"https:\/\/genoinfocore.crc.nycu.edu.tw\/?p=230"},"modified":"2025-06-30T03:22:33","modified_gmt":"2025-06-30T03:22:33","slug":"rna%e5%93%81%e8%b3%aa%e5%88%86%e6%9e%90","status":"publish","type":"page","link":"https:\/\/genoinfocore.crc.nycu.edu.tw\/?page_id=230","title":{"rendered":"RNA\u54c1\u8cea\u5206\u6790"},"content":{"rendered":"<h2 class=\"wp-block-post-title\">RNA\u54c1\u8cea\u5206\u6790<\/h2>\n\n\n<hr class=\"wp-block-separator has-text-color has-vivid-red-color has-alpha-channel-opacity has-vivid-red-background-color has-background is-style-wide\"\/>\n\n\n\n<p class=\"wp-block-paragraph\">\u70ba\u4e86\u907f\u514d\u4e0d\u5fc5\u8981\u7684\u6d6a\u8cbb\uff0c\u672c\u4e2d\u5fc3\u5f37\u70c8\u5efa\u8b70\u7533\u8acb\u8005\u81ea\u884c\u6aa2\u6e2cRNA\u54c1\u8cea\uff0cRNA\u54c1\u8cea\u662f\u6676\u7247\u5206\u6790\u6b63\u78ba\u6027\u6700\u5927\u7684\u5f71\u97ff\u56e0\u5b50\u4e4b\u4e00\uff0c\u932f\u8aa4\u7684\u6578\u64da\u6bd4\u6c92\u6709\u6578\u64da\u66f4\u4ee4\u4eba\u982d\u75db\u3002 \u672c\u4e2d\u5fc3\u8981\u6c42\u7533\u8acb\u4eba\u81f3\u5c11\u5b8c\u6210\u5169\u9805\u6e2c\u8a66\uff08\u7d14\u5ea6\uff1aOD260\/280 measurement\uff0c\u5b8c\u6574\u6027\uff1aElectrophoresis or Bioanalyzer test\uff09\uff0c\u672c\u4e2d\u5fc3\u4ea6\u6703\u9032\u884c\u91cd\u8907\u6e2c\u8a66\uff0c\u4e26\u4f9d\u64da\u4ee5\u4e0b\u6a19\u6e96\u5224\u5b9aRNA\u7684\u54c1\u8cea\uff1a<br><br><strong>1. RNA\u7d14\u5ea6<\/strong><br>RNA\u5728\u7d14\u5316\u904e\u7a0b\u4e2d\uff0c\u5bb9\u6613\u53d7\u5230DNA\u3001\u86cb\u767d\u8cea\u53ca\u6709\u6a5f\u6eb6\u5291\u7684\u5f71\u97ff\uff0c\u9019\u4e9b\u6b98\u5b58\u7269\u5c07\u6703\u5f71\u97ff\u5f80\u5f8c\u7684\u64cd\u4f5c\u3002\u5149\u8b5c\u5206\u6790\u5229\u7528\u7269\u8cea\u5c0d\u4e0d\u540c\u6ce2\u9577\u5149\u7684\u5438\u6536\u5ea6\u7684\u4e0d\u540c\uff0c\u53ef\u4ee5\u9451\u5225\u51fa\u6eb6\u6db2\u7d14\u5ea6\u53ca\u6fc3\u5ea6\u3002\u672c\u4e2d\u5fc3\u6703\u5c0dRNA\u6a23\u54c1\u505aOD measurement\u7d14\u5ea6\u6aa2\u6e2c\uff0c\u540c\u6642\u4e5f\u8981\u6c42\u7533\u8acb\u4eba\u5728\u9001\u4ef6\u524d\u81ea\u884c\u6aa2\u6e2c\uff0c\u672c\u4e2d\u5fc3QC\u7684\u6a19\u6e96\u5982\u4e0b\u5716\u6240\u793a\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"536\" height=\"201\" src=\"https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/\u57169.jpg\" alt=\"\" class=\"wp-image-161\" srcset=\"https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/\u57169.jpg 536w, https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/\u57169-300x113.jpg 300w\" sizes=\"(max-width: 536px) 100vw, 536px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><br><strong>2. RNA\u5b8c\u6574\u6027<\/strong><br>\u9664\u4e86\u7d14\u5ea6\u4ee5\u5916\uff0cRNA\u7684\u5b8c\u6574\u6027\u4e5f\u662f\u975e\u5e38\u7684\u91cd\u8981\uff0c\u81ea\u7136\u754c\u7121\u6240\u4e0d\u5728\u7684RNase\uff0c\u4f7f RNA\u4fdd\u5b58\u76f8\u7576\u4e0d\u6613\uff0c\u5728\u904b\u9001\u53ca\u4fdd\u5b58\u7684\u904e\u7a0b\u4e2d\u5982\u679c\u906d\u53d7\u6c61\u67d3\uff0cRNA\u6703\u5f88\u5feb\u7684\u906d\u5206\u89e3\u7834\u58de\uff0c\u70ba\u4e86\u78ba\u4fdd\u5be6\u9a57\u7684\u6578\u64da\u4e0d\u53d7\u5f71\u97ff\uff0c\u672c\u4e2d\u5fc3\u6703\u5c0dRNA\u6a23\u54c1\u4f5c\u5b8c\u6574\u6027\u7684\u6aa2\u6e2c\uff0c\u540c\u6642\u4e5f\u8981\u6c42\u7533\u8acb\u4eba\u5728\u9001\u4ef6\u524d\u81ea\u884c\u6aa2\u6e2c\u3002\u76ee\u524d\u672c\u4e2d\u5fc3\u7684\u505a\u6cd5\u6709\u5169\u7a2e\uff0c(1)\u96fb\u6cf3\u6cd5\uff0c(2)\u6d41\u5f0f\u6676\u7247\uff082100 Bioanalyzer\uff09\uff0c\u5176\u6a19\u6e96\u5982\u4e0b\uff1a<br><br><strong>Integrity test by electrophoresis<\/strong><br>\u53d61.5\u03bcg RNA\u8dd1<a href=\"https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/Molbio_Denatured-Electrophoresis-for-RNA.doc\" data-type=\"link\" data-id=\"https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/Molbio_Denatured-Electrophoresis-for-RNA.doc\">denature electrophoresis<\/a>\uff0cRNA\u61c9\u51fa\u73fe\u6e05\u695a\u7684\u5169\u689dBand\uff0828S\uff0f18S rRNA\uff09\uff0c\u6709\u6642\u6703\u6709\u7b2c\u4e09\u689dBand\uff085S rRNA\uff09\uff0c\u6700\u4e0a\u65b9\u4e0d\u53ef\u51fa\u73feDNA\u6c61\u67d3\uff08\u898bLane 4\uff09\uff0c28S\uff0f18S\u61c9\u8fd1\u4f3c\u5169\u500d\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"763\" height=\"287\" src=\"https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/\u571610.jpg\" alt=\"\" class=\"wp-image-162\" srcset=\"https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/\u571610.jpg 763w, https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/\u571610-300x113.jpg 300w\" sizes=\"(max-width: 763px) 100vw, 763px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Integrity test by Bioanalyzer<\/strong><br>\u50b3\u7d71\u7684Denature electrophoresis\u53ef\u4ee5\u6e05\u695a\u5206\u6790RNA\u7684\u5b8c\u6574\u6027\uff0c\u7136\u800c\u6700\u5927\u7684\u554f\u984c\u662f\u9700\u89811.5\u03bcg RNA\uff0c\u5c0d\u65bc\u8a31\u591a\u73cd\u8cb4\u81e8\u5e8a\u6a23\u54c1\u5f80\u5f80\u7d93\u4e0d\u8d77\u9019\u6a23\u7684\u6d88\u8017\uff0c\u800c\u5fae\u6d41\u6676\u7247\u6280\u8853\uff082100 Bioanalyzer, agilent inc.\uff09\u53ef\u4ee5\u6539\u5584\u9019\u6a23\u7684\u554f\u984c\uff0c\u5229\u7528\u5fae\u578b\u5316\u4f7f\u6a23\u54c1\u9700\u6c42\u964d\u523025ng\uff0c\u672c\u4e2d\u5fc3\u63a1\u7528\u6b64\u6280\u8853\u9032\u884cRNA\u5b8c\u6574\u6027\u5206\u6790\uff0c\u5982\u540c\u50b3\u7d71\u96fb\u6cf3\uff0c\u9664 28S\u300118S\u5916\u4e0d\u61c9\u6709\u5176\u5b83\u5cf0\u503c\uff0c28S\uff0f18S\u61c9\u57281.8~2.1\u4e4b\u9593\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"560\" height=\"897\" src=\"https:\/\/genoinfocore.crc.nycu.edu.tw\/wp-content\/uploads\/2025\/06\/\u571611.gif\" alt=\"\" class=\"wp-image-163\"\/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u70ba\u4e86\u907f\u514d\u4e0d\u5fc5\u8981\u7684\u6d6a\u8cbb\uff0c\u672c\u4e2d\u5fc3\u5f37\u70c8\u5efa\u8b70\u7533\u8acb\u8005\u81ea\u884c\u6aa2\u6e2cRNA\u54c1\u8cea\uff0cRNA\u54c1\u8cea\u662f\u6676\u7247\u5206\u6790\u6b63\u78ba\u6027\u6700\u5927\u7684\u5f71\u97ff\u56e0\u5b50\u4e4b\u4e00\uff0c\u932f [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-230","page","type-page","status-publish","hentry"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/230","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=230"}],"version-history":[{"count":13,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/230\/revisions"}],"predecessor-version":[{"id":406,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/230\/revisions\/406"}],"wp:attachment":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=230"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}