{"id":226,"date":"2025-06-25T02:27:02","date_gmt":"2025-06-25T02:27:02","guid":{"rendered":"https:\/\/genoinfocore.crc.nycu.edu.tw\/?p=226"},"modified":"2025-06-30T03:27:29","modified_gmt":"2025-06-30T03:27:29","slug":"%e5%b8%b8%e8%a6%8b%e5%95%8f%e9%a1%8c","status":"publish","type":"page","link":"https:\/\/genoinfocore.crc.nycu.edu.tw\/?page_id=226","title":{"rendered":"\u5e38\u898b\u554f\u984c"},"content":{"rendered":"

\u5e38\u898b\u554f\u984c<\/h2>\n\n\n
\n\n\n\n

About sample preparation and quality check<\/strong>
1. \u8acb\u554f\u6838\u5fc3\u5be6\u9a57\u5ba4\u662f\u5426\u63a5\u53d7RNA\u4ee5\u5916\u7684\u6a23\u54c1\u9001\u4ef6\uff1f<\/strong> <\/em>
\u7b54\uff1a\u5c0d\u4e0d\u8d77\uff0c\u76ee\u524d\u6838\u5fc3\u5be6\u9a57\u5ba4\u4e26\u6c92\u6709\u8db3\u5920\u7684\u64cd\u4f5c\u80fd\u91cf\uff0c\u53ef\u4ee5\u63a5\u53d7RNA\u4ee5\u5916\u7684\u6a23\u54c1\uff08\u4f8b\u5982\uff1a\u51b7\u51cd\u7d44\u7e54\u53ca\u81d8\u584a\uff09\u3002RNA\u62bd\u53d6\u7684\u65b9\u6cd5\u53ca\u7522\u91cf\u6b67\u7570\u6027\u975e\u5e38\u7684\u5927\uff0c\u6838\u5fc3\u5be6\u9a57\u5ba4\u4e26\u6c92\u6709\u4e00\u500b\u53ef\u4ee5\u9069\u7528\u65bc\u6240\u6709\u6a23\u54c1\u7684\u7a0b\u5e8f\uff0c\u53ef\u4ee5\u78ba\u5b9a\u5f97\u5230\u6700\u4f73\u6548\u679c\uff0c\u6211\u5011\u8a8d\u70ba\uff0c\u6211\u5011\u4e0d\u6703\u6bd4\u7533\u8acb\u4eba\u66f4\u4e86\u89e3\u6a23\u54c1\u7684\u7279\u6027\uff0c\u540c\u6642\u4e5f\u6c92\u6709\u8db3\u5920\u7684\u6642\u9593\u53ca\u4eba\u529b\u91dd\u5c0d\u6bcf\u4e00\u500b\u6a23\u54c1\u53bb\u5617\u8a66\u3002\u6240\u4ee5\u5728\u73fe\u968e\u6bb5\u7684\u4f5c\u696d\u4e2d\uff0c\u6211\u5011\u4e26\u4e0d\u63a5\u53d7RNA\u4ee5\u5916\u7684\u6a23\u54c1\u3002

2. \u5982\u4f55\u5224\u5b9aRNA\u7684\u54c1\u8cea\u662f\u5426\u5408\u683c\uff1f<\/em><\/strong>
\u7b54\uff1a\u8acb\u53c3\u95b1RNA\u54c1\u8cea\u5206\u6790<\/a>\u3002<\/p>\n\n\n\n

3. \u8840\u6db2\u4e2d\u542b\u6709\u591a\u5c11\u767d\u8840\u7403\uff1f\u53ef\u62bd\u51fa\u591a\u5c11DNA\u3001RNA\u548cprotein\uff1f<\/em><\/strong>
\u7b54\uff1a\u5168\u8840\u53ef\u5206\u70ba\u8840\u6f3f\u8207\u8840\u7403\u5169\u90e8\u5206\uff0c\u5404\u4f54\u8840\u6db2\u768455%\u820745%\u3002\u8840\u7403\u53c8\u53ef\u5206\u70ba\u7d05\u8840\u7403\uff08RBC\uff09\u3001\u767d\u8840\u7403\uff08WBC\uff09\uff0c\u8207\u8840\u5c0f\u677f\uff0c\u5176\u6240\u4f54\u6bd4\u4f8b\u70ba500:1:30\u3002\u8840\u6db2\u4e2dWBC\u7684\u6578\u91cf\u6703\u96a8\u5e74\u9f61\u3001\u6027\u5225\u7b49\u751f\u7406\u72c0\u614b\u800c\u7570\uff0c\u6b63\u5e38\u6210\u4eba\u7684WBC\u53c3\u8003\u6578\u503c\u70ba4500~11000\/ml\uff0c2\u6b72\u4ee5\u524d\u4e4b\u5b69\u7ae5\u7684WBC\u53c3\u8003\u6578\u503c\u5247\u70ba5000~13000\/ml\u3002\u5168\u8840\u4e2d\u6240\u542b\u6709DNA\u542b\u91cf\u70ba30~60 mg\/ml blood (6 pg\/cell)\uff0cRNA\u542b\u91cf\u70ba1~5 mg\/ml blood\uff0cplasma protein\u542b\u91cf\u70ba60~80 mg\/ml blood\u3002DNA\u7d14\u5316\u65b9\u5f0f\u53ef\u5229\u7528QIAGEN\u4e4bQIAamp DNA Blood Mini Kit\uff0c\u7522\u91cf\u7d04\u70ba4~12 mg DNA\/200ml healthy whole blood\uff0cRNA\u7d14\u5316\u65b9\u5f0f\u53ef\u5229\u7528QIAGEN\u4e4bQIAamp RNA Blood Mini Kit\uff0c\u7522\u91cf\u7d04\u70ba1~5 mg RNA \/ml healthy whole blood\u3002

\u53c3\u8003\u6587\u737b
1. \u4f55\u654f\u592b\u7de8\u8457\uff0c\u8840\u6db2\u5b78\u7b2c\u4e09\u7248\uff0c\u8840\u6db2\u5e38\u898f\u6aa2\u67e5\uff0c2002\uff0cp583-616
2. http:\/\/www1.qiagen.com\/

4. 106<\/sup> cells\u53ef\u62bd\u5230\u591a\u5c11RNA\uff1f\u53ef\u505a\u591a\u5c11cDNA\uff1f<\/strong> <\/em>
\u7b54\uff1a106<\/sup> cells\u53ef\u62bd\u523010~35\u03bcg total RNA\uff0c\u5982\uff1aNIH\/3T3\u53ef\u62bd\u53d6\u523010\u03bcg total RNA\uff0cHela\u53ef\u62bd\u53d6\u523015\u03bcg total RNA\uff0cCOS-7\u53ef\u62bd\u53d6\u523035\u03bcg total RNA\uff0cLMH\u53ef\u62bd\u53d6\u523012\u03bcg total RNA\uff0cHuh\u53ef\u62bd\u53d6\u523015\u03bcg total RNA\uff1b\u81f3\u65bc\u53ef\u8f49\u6210\u591a\u5c11cDNA\uff0c\u5f97\u4f9d\u57fa\u56e0\u5728\u7d30\u80de\u4e2d\u7684\u8868\u73fe\u60c5\u6cc1\u800c\u5b9a\uff0c\u5982\uff1aAbundant\uff08GAPD\u3001\u03b2-Actin\u2026\uff09\u70ba100~1000 copies\/cell\uff0cIntermediate\uff08\u5982\uff1aTranslocation factors\uff09\u70ba10~100 copies\uff0cRare\uff08\u5982\uff1aTNF\u3001M-CSF\uff09\u70ba<10 copies\/cell\u3002\u4ee5NIH\/3T3\u70ba\u4f8b106<\/sup> cells\u53ef\u67097*108<\/sup>~7*109<\/sup> copies\u7684GAPD\u30017*107<\/sup>~7*108<\/sup> copies\u7684translocation factors\u30017*106<\/sup> copies\u7684TNF\u3002

About affymetrix genechip service<\/strong>
1. \u4ec0\u9ebc\u662fTest Chip\uff1f<\/em><\/strong>
\u7b54\uff1aTest\u662f\u96c6\u5408\u4e86\u8a31\u591a\u7269\u7a2e\u7684house keeping gene\u7684\u5c0f\u578bchips\uff0c\u7528\u9014\u662f\u5728\u78ba\u8a8d RNA\u7684\u54c1\u8cea\u4ee5\u53ca\u6a23\u54c1\u8655\u7406\u6642\u6709\u7121\u7455\u73bc\u3002\u56e0\u70ba\u6676\u7247\u6210\u672c\u5f88\u9ad8\uff0c\u6211\u5011\u5e0c\u671b\u6bcf\u4e00\u6b21\u90fd\u80fd\u6709\u6700\u597d\u7684\u7d50\u679c\uff0c\u7136\u800c\u8a31\u591a\u6a23\u54c1\u6216\u64cd\u4f5c\u4e0a\u7684\u554f\u984c\uff0c\u5fc5\u9808\u8981\u5728\u5be6\u9a57\u5f8c\u6383\u63cf\u624d\u6703\u767c\u73fe\uff0c\u7136\u800c\u90a3\u6642\u5df2\u7121\u6cd5\u633d\u6551\u4e86\uff0c\u56e0\u6b64\uff0c\u9019\u6642\u6211\u5011\u6703\u5efa\u8b70\u5148\u4ee5\u6210\u672c\u8f03\u4f4e\u7684test chip \u6e2c\u8a66\uff0c\u82e5test chip\u7d50\u679c\u6b63\u78ba\uff0c\u518d\u9032\u884c\u6b63\u5f0f\u5be6\u9a57\u3002\u672c\u4e2d\u5fc3\u4f7f\u7528\u4efb\u4f55\u4e00\u7a2echip\u6642\uff0c\u7686\u53ef\u4ee5\u984d\u5916\u7533\u8acb\u4f7f\u7528test chip\uff0c\u50c5\u9700\u8ca0\u64d4chip\u6210\u672c\u50f9\u683c\u3002<\/p>\n\n\n\n

2. \u6211\u77e5\u9053\u6211\u7684RNA\u5f88\u721b\uff0c\u53ef\u662f\u6211\u975e\u505a\u4e0d\u53ef<\/em><\/strong>\uff01
\u7b54\uff1a\u6709\u6642\u6a23\u54c1\u53d6\u5f97\u975e\u5e38\u56f0\u96e3\uff0c\u800c\u4e14\u54c1\u8cea\u4e0a\u7121\u6cd5\u9054\u5230\u8981\u6c42\uff0c\u9019\u4e00\u9ede\u6211\u5011\u53ef\u4ee5\u5b8c\u5168\u7406\u89e3\uff01\u9019\u6642\u6211\u5011\u6703\u5efa\u8b70\u60a8\u8dd1\u4e00\u7247test chip\uff0c\u6e2c\u8a66\u5f9e\u6a23\u54c1\u53d6\u5f97\u6578\u64da\u7684\u53ef\u884c\u6027\uff0c\u518d\u505a\u6c7a\u5b9a\u3002<\/p>\n\n\n\n

3. \u6211\u60f3\u505a\u7684chip\u6c92\u6709\u5728\u670d\u52d9\u7684\u9805\u76ee\u4e4b\u4e2d\uff1f<\/em><\/strong>
\u7b54\uff1a\u53ea\u8981\u662feukaryotic expression\u7684chip\uff0c\u6211\u5011\u90fd\u53ef\u4ee5\u8655\u7406\uff0c\u6211\u5011\u4e5f\u53ef\u4ee5\u70ba\u60a8\u4ee3\u8a02\uff0c\u4e0d\u904e\u56e0\u70ba\u91cf\u5c11\uff0c\u6240\u4ee5\u5728\u6676\u7247\u6210\u672c\u4e0a\u6703\u9ad8\u51fa\u5f88\u591a\uff0c\u56e0\u6b64\u8acb\u9023\u7d61\u6211\u5011\uff0c\u6211\u5011\u6703\u70ba\u60a8\u8a62\u50f9\uff0c\u518d\u544a\u77e5\u53ef\u80fd\u7684\u670d\u52d9\u65b9\u6cd5\u53ca\u50f9\u683c\u3002<\/p>\n\n\n\n

About real-time quantitative PCR service<\/strong>
1. \u8acb\u554f\u59d4\u8a17\u55ae\u4f4d\u662f\u5982\u4f55\u5b9a\u7fa9\uff1f<\/em><\/strong>
\u7b54\uff1a\u7531\u65bc\u672c\u5be6\u9a57\u5ba4\u4f7f\u7528\u7684\u662f384well plate\uff0c\u56e0\u6b64\u6709\u4e9b\u5be6\u9a57\u8017\u6750\u53ca\u4eba\u5de5\u6210\u672c\u662f\u4ee5384\u53cd\u61c9\u70ba\u55ae\u4f4d\uff0c\u4ea6\u5373\u6bcf\u4e00\u6b21\u4e0d\u8ad61~384\u500b\u53cd\u61c9\u5747\u9700\u4f7f\u7528\u4e00\u7247\u5c01\u819c\u8ddf\u4e00\u500b384well plate\uff0c\u6240\u4ee5\u7406\u8ad6\u4e0a\u9700\u4ee5384\u500b\u53cd\u61c9\u70ba\u4e00\u500b\u8a08\u8cbb\u55ae\u4f4d\uff08\u570b\u5bb6\u578b\u8a08\u756bSYBR Green\u61c9\u70baNT$1080\uff09\uff0c\u4f46\u8003\u91cf\u5230\u5ba2\u6236\u7684\u6210\u672c\u8ca0\u64d4\uff0c\u56e0\u6b64\u4ee5192\u500b\u53cd\u61c9\u70ba\u4e00\u500b\u8a08\u8cbb\u55ae\u4f4d\uff08\u570b\u5bb6\u578b\u8a08\u756bSYBR Green\u61c9\u70baNT$540\uff09\uff1b\u6240\u4ee5\u7e3d\u53cd\u61c9\u6578\u9664\u4ee5192\u5c3e\u6578\u7121\u689d\u4ef6\u9032\u4f4d\u81f3\u6574\u6578\uff0c\u5373\u70ba\u59d4\u8a17\u55ae\u4f4d\u3002<\/p>\n\n\n\n

\u8209\u4f8b\u4f86\u8aaa\uff1a
100\u500b\u53cd\u61c9==\u59d4\u8a17\u55ae\u4f4d\u70ba1
192\u500b\u53cd\u61c9==\u59d4\u8a17\u55ae\u4f4d\u70ba1
193\u500b\u53cd\u61c9==\u59d4\u8a17\u55ae\u4f4d\u70ba2
384\u500b\u53cd\u61c9==\u59d4\u8a17\u55ae\u4f4d\u70ba2
385\u500b\u53cd\u61c9==\u59d4\u8a17\u55ae\u4f4d\u70ba3<\/p>\n\n\n\n

2. \u9032\u884cQ-PCR\u9700\u8981\u591a\u5c11RNA\uff1f<\/em><\/strong>
\u7b54\uff1a\u9032\u884cQ-PCR\u9700\u898110~100 ng RNA\/reaction\u3002<\/p>\n\n\n\n

\u53c3\u8003\u8cc7\u6599\u4f86\u6e90\uff1a
1. QIAGEN catalog-Rneasy Mini Handbook, OneStep RT-PCR Kit Handbook\u3002<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

About sample preparation and quality check1. \u8acb\u554f\u6838\u5fc3\u5be6\u9a57\u5ba4\u662f\u5426\u63a5 […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-226","page","type-page","status-publish","hentry"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/226","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=226"}],"version-history":[{"count":7,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/226\/revisions"}],"predecessor-version":[{"id":412,"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/226\/revisions\/412"}],"wp:attachment":[{"href":"https:\/\/genoinfocore.crc.nycu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=226"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}